Master Thesis in Biochemie - Modelling the molecular role of PRDM16 in cardiomyocytes
Charité - Universitätsmedizin Berlin, Experimental and Clinical Research Center (ECRC)
The Experimental and Clinical Research Center (ECRC), Berlin-Buch is a cooperation between the Charité - Universitätsmedizin Berlin and the Max-Delbrück-Centrum for Molecular Medicine (MDC) that focuses on biomedical translational research. The ECRC research group Genetics of Congenital Heart Disease conducts translational research to explore disease mechanisms and potential therapeutic options of pediatric cardiomyopathy.
Master Thesis in Biochemistry | Biology | Biotechnology | Molecular Medicine
Modelling the molecular role of PRDM16 in cardiomyocytes
in Berlin, Germany
Background Mutation of the transcriptional regulator PR domain containing 16 (PRDM16) causes cardiomyopathy. To further delineate the role of PRDM16 in cardiomyocytes, we investigate the molecular mechanism of the PRDM16 associated cardiomyopathy in human induced pluripotent stem cells (hiPSC) and hiPSC derived cardiomyocytes (hiPSC-CM). Currently we assess metabolic alterations after PRDM16 deactivation in hiPSC-CM.
Maintenance of hiPSC and differentiation of hiPSC-CM
Metabolic measurements in hiPSC-CM
Analysis of protein expression and metabolite levels in hiPSC-CM
Fulfilled requirements for a master thesis
First laboratory expertise with basic techniques in molecular biology, protein biochemistry and cell biology
High motivation for a biomedical research project
Please email your complete application including CV, bachelor certificate, other professional degrees,
and special qualifications.
Dr. rer. nat. Jirko Kühnisch
Charité - Universitätsmedizin Berlin
Experimental and Clinical Research Center (ECRC)
Lindenberger Weg 80
13125 Berlin, Germany