We are looking for a highly motivated Master's project student, with a background in cell biology,
biochemistry, immunology, molecular biology, biotechnology or other relevant fields.
Background: Immunotherapy with T cells engineered to recognize cancer cells via chimeric antigen
receptors (CAR T cells) has shown impressive clinical efficacy against leukemias. A major barrier in solid
tumors is the lack of T cell trafficking and penetration into the tumor microenvironment (TME). This
project develops an innovative strategy to amplify the anti-tumor activity of gene-engineered T cells
by integrated secretion of antagonist against receptors expressed by cells in the TME. We hypothesize
local modulation of these cells will allow further T cell infiltration and activation, resulting in tumor
Aim: The goal of this Master's project is to prove the production and functionality of anti-TME scFvs in
a cell line model and then transfer it to CAR T cells.
Experimental procedures: The student will produce anti-TME scFvs in HEK293T cells by using already
established cell culture methods (transfection, viral transduction). The scFvs can be detected using
Western Blot, FACS, ELISA and qPCR methods. Furthermore, the student will perform functional in vitro
studies (e.g. proliferation and migration assays) to prove their functionality.
Prof. Claudia Rössig, Dr. Bianca Altvater, and PhD student Lina Pérez Lanuza will supervise the project.
The starting date will be as soon as possible or after agreement.
Please send your application to Lina Pérez Lanuza (
application should include a few lines about yourself and your interests, a transcript of grades, and a